Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation

Acta Cirurgica Brasileira

Natasha Machado¹  Silvio Eduardo Duailibi¹  Jennifer Adriane Dos Santos¹  Vanessa Penna¹  Lydia Masako Ferreira¹  Monica Talarico Duailibi¹ 

关键词: Dental Pulp;    Culture Media;    Glucose;    Glutamine;    Regenerative Medicine;   

DOI  :  10.1590/S0102-8650201400160006

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate microscopic behavior and viability of dental pulp stem cells under glucose and glutamine deprivation. METHODS: Human tooth tissues were minced in isolated pieces and cultured until the desired cellular proliferation for experimental phases. Cells were cultured under variations of glucose and glutamine in both serum presence and absence, and then those cells were evaluated according to number and viability by MTT assay. The confocal microscopy analyzed cytoskeleton, nucleus, and mitochondria integrity. RESULTS: A low concentration of glucose favored cellular viability and microscopic behavior; the presence of glutamine in culture medium was favorable only when associated with glucose. The cellular biological potential in culture could be preserved in serum absence if nutritional requirements are adequate. CONCLUSION: Cell microscopic behavior and viability have demonstrated better patterns on serum-free low glucose culture medium with glutamine deprivation.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License