Animal model of chronic kidney disease using a unilateral technique of renal ischemia and reperfusion in White New Zealand rabbits

Acta Cirurgica Brasileira

Eliane Barbosa Togoe¹  Iandara Schettert Silva¹  Juliana Loprete Cury¹  Albert Schiaveto Souza¹  José Henrique Saraiva Borges¹  Klaus Casaro Saturnino¹ 

关键词: Renal Insufficiency;    Chronic;    Models;    Animal;    Rabbits;   

DOI  :  10.1590/S0102-8650201400160005

来源: SciELO

【 摘 要 】

To establish a model of chronic kidney disease in White New Zealand rabbits, using an exclusive unilateral technique of renal ischemia and reperfusion. Twenty males White New Zealand rabbits were used. All animals were subjected to the following: pre-surgical blood collection (1^st collection) for creatinine and urea serum analysis, left renal ischemia and reperfusion surgery technique, another blood sample was collected after 6 weeks post surgery (2^nd collection), the last blood sample (3^rdcollection) blood sample was taken 11 weeks post surgery (pre-euthanasia), euthanasia and withdrawal of right and left kidney for histopathological analysis. The creatinine levels after surgery was statistically significant higher in the 3^rd collection, regarding the 1^st and 2^ndcollection (p<0.05). A significant statistic increase for urea was showed only in the 2^nd collection (p<0.05) when compared to the 1^stand 3^rd collections. Histopathological analysis showed bilateral lesions in the renal tissue, consistent to the process of ischemia and reperfusion. This exclusive unilateral technique of renal ischemia and reperfusion without nephrectomy in White New Zealand rabbits, showed effectiveness in getting an animal model of chronic kidney disease

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