Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis

Acta Cirurgica Brasileira

Felipe Mendonça Chaim¹  Daniela Tiemi Sato¹  Murilo Rocha Rodrigues¹  Alice Moreira Dias¹  Paulo Pedroso Silveira Júnior¹  José Aires Pereira¹  Carlos Augusto Real Martinez¹ 

关键词: Colitis;    Colostomy;    Fatty Acids;    Volatile;    Mucins;    Sucralfate;    Image Processing;    Computer-Assisted;    Rats;   

DOI  :  10.1590/S0102-8650201400150001

来源: SciELO

【 摘 要 】

PURPOSE:To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis.METHODS: Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p<0.05).RESULTS: Enemas containing sucralfate improves the inflammation and increases the tissue contents of neutral and acid mucins. The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention.CONCLUSIONS:Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream.

【 授权许可】

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