Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin

Acta Cirurgica Brasileira

Erica Calcagno Raymundo¹  Bernardo Hochman¹  Michele Akemi Nishioka¹  José Octávio Gonçalves De Freitas¹  Jéssica Ruivo Maximino¹  Gerson Chadi¹  Lydia Masako Ferreira¹ 

关键词: Carbon Dioxide;    Substance P;    Calcitonin Gene-Related Peptide;    Neurogenic Inflammation;    Skin;    Rats;   

DOI  :  10.1590/S0102-86502014000400002

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin.METHODS: Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB).RESULTS: In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups.CONCLUSION: Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin Gene-Related Peptide (15 kDa) neuropeptides in rat skin.

【 授权许可】

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