Acta Cirurgica Brasileira | |
Oxidative stress and fatty acid profile in Wistar rats subjected to acute food restriction and refeeding with high-fat diets | |
Ana Lígia Da Silva Nassar1  Luisa Pereira Marot1  Paula Payão Ovidio1  Gabriela Salim Ferreira De Castro1  Alceu Afonso Jordão Júnior1  | |
关键词: Fasting; Diet; High-Fat; Oxidative Stress; Fatty Acids; Rats; | |
DOI : 10.1590/S0102-86502014000300006 | |
来源: SciELO | |
【 摘 要 】
PURPOSE: To assess oxidative stress and the profile of fatty acids incorporated into the hepatic tissue of animals refed with high-fat (HF) diets after acute food restriction.METHODS: Fifty male Wistar rats were divided into five groups and fasting for 48 hours. One group was sacrificed without refeeding (NR), a control group (C) was refed with the standard AIN-93 diet and the remaining groups with HF diets respectively consisting of hydrogenated vegetable oil (PHVO), trans-free (TF) margarine and trans-free margarine enriched with ω-3 and ω-6 (O). After this period the animals were sacrificed for malondialdehyde (MDA), catalase and hepatic fatty acid determination.RESULTS: The groups refed with HF diets showed elevation of MDA levels compared to the C group (p<0.001 for GVH and p<0.01 for TF and O). Hepatic catalase activity was higher in the TF and O groups compared to group C (p<0.05 for both). The amount of saturated fatty acids was lower in the PHVO and O groups compared to the remaining ones (p<0.001).CONCLUSION: The consumption of high-fat diets after prolonged fasting favors oxidative imbalance in hepatic tissue.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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