Median lethal needle caliber in two models of experimental sepsis

Acta Cirurgica Brasileira

Armando José D'acampora¹  Giovani De Figueiredo Locks¹ 

关键词: Sepsis;    Disease Models;    Animal;    Rats;   

DOI  :  10.1590/S0102-86502014000100001

来源: SciELO

【 摘 要 】

PURPOSE: To estimate the median lethal needle caliber (LC50) of a new experimental sepsis model and compare it to the LC50 of the cecal ligation and puncture (CLP) sepsis model.METHODS: Male albino Wistar rats were studied (n=22). Animals were allocated into two study groups. In Group I, experimental sepsis was induced by cecal ligation and puncture. In Group II, experimental sepsis was induced by ascending colon ligation and cecal puncture. Up-and-down method was used to determinate the LC50.RESULTS: LC50 in Group I was 19 Gauge (Confidence Interval 17 to 22 Gauge). Determination of LC50 was not possible in Group II due to the death of all animals.CONCLUSION: LC50 in cecal ligation and puncture is 19 Gauge. The lethality of the new model tested in this trial is very high.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License