Immunoexpression of proliferating cell nuclear antigen (PCNA) in spleen of splenectomized rats with preservation of inferior pole, submitted to hyperbaric oxygenation

Acta Cirurgica Brasileira

Marcela Souza Lima Paulo²  Filipy Taifson Barbosa Dos Santos¹  Polyanna Gonçalves Rocha¹  Mirella De Barros Silva¹  Luiz Cálice Cintra¹  Luciene Lage Da Motta¹  Flávia Imbroisi Valle Errera¹  Danilo Nagib Salomão Paulo¹  Tarcizo Afonso Nunes¹ 

[1] ,Federal University of Minas Gerais Postgraduate Program in Surgical and Ophthalmological Applied Sciences Belo Horizonte MG ,Brazil

关键词: Proliferating Cell Nuclear Antigen;    Hyperbaric Oxygenation;    Splenectomy;    Rats;   

DOI  :  10.1590/S0102-86502013001000001

来源: SciELO

【 摘 要 】

PURPOSE: To analyze PCNA immunoexpression on the inferior pole of the spleen of splenectomized rats submitted to hyperbaric oxygenation (HBO). METHODS: Were analyzed fragments of the inferior pole of the spleen of 20 male Wistar rats submitted to splenectomy with preservation of the inferior pole. The rats were divided in two groups: group A (n=10) without HBO and group B (n=10) submitted to HBO at 2, 5 atmospheres per 120 minutes, twice a day for three days and once a day for seven days. The groups were then subdivided in four subgroups: A15 (n=5), with euthanasia on the 15th day; A45 (n=5), with euthanasia on the 45th day; B15 (n=5) with euthanasia on the 15th day and B45 with euthanasia on the 45th day. Respectively on these days, fragments of the inferior pole of the spleen of all animals were collected and analyzed with the immunohistochemistry technique in order to evaluate PCNA expression. RESULTS: There was an expressive increase in PCNA immunoreactivity in the group B. The 45 day postoperative period resulted in a higher level of positivity than the 15 day postoperative period (p<0.01). CONCLUSION: The quantitative analysis of proliferating cell nuclear antigen positive suggests that hyperbaric oxygenation increases cellular proliferation, contributing to splenic regeneration.

【 授权许可】

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