Superoxid dismutase activity in portal vein endothelium after partial liver resection

Acta Cirurgica Brasileira

Rogério Heggendorn Sayão Filho²  Marcos Vinicius Perini¹  José Arnaldo Cruz²  Juliana Requena²  Hermes Vieira Barbeiro¹  Nilza Trindade Molan¹  Fabio Pinatel Lopasso¹  Luiz Augusto Carneiro D'albuquerque¹  Ivan Cecconello¹ 

[1] ,University of Sao Paulo Medical School ,Brazil

关键词: Hepatectomy;    Oxidative Stress;    Malondialdehyde;    Superoxide Dismutase;    Rats;   

DOI  :  10.1590/S0102-86502013000900004

来源: SciELO

【 摘 要 】

PURPOSE: To investigate superoxide dismutase (SOD) activity in the portal vein endothelium and malondialdehyde acid (MDA) production in liver tissue of rats submitted to 70% hepatectomy. METHODS:Twelve rats were distributed in two groups (hepatectomy and sham). Animals were sacrificed on post operative day 1 and portal vein, liver tissue and blood samples were collected. Portal vein SOD production was measured using lucigenin-amplified chemiluminescence assays. MDA measurement was used as an index of oxidative stress through the formation of TBARS (Thiobarbituric Acid Reactive Species). RESULTS: There was no difference in post operative bilirrubin, AST, ALT levels between groups. DHL level was higher in the hepatectomy group (p=0.01). MDA production in the remnant liver tissue and endothelial portal vein SOD activity were also significantly (p<0.05) elevated in the hepatectomy group when compared to control group. There was no correlation between MDA and SOD activity. SOD activity, on the other hand, showed a positive correlation with LDH level (p=0.038) and MDA levels showed a positive correlation with AST and ALT levels (p<0.001). CONCLUSION: There is an increased production of malondialdehyde acid in liver tissue after partial hepatectomy and increased activity of superoxide dismutase in portal vein endothelium as well.

【 授权许可】

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