In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells

Acta Cirurgica Brasileira

Oduvaldo Câmara Marques Pereira-junior¹  Sheila Canevese Rahal²  João Ferreira Lima-neto¹  Fernanda Da Cruz Landim-alvarenga¹  Frederico Ozanan Barros Monteiro¹ 

[1],UNESP School of Veterinary Medicine and Animal Science Department of Veterinary Surgery and AnesthesiologyBotucatu SP

关键词: Hydroxyapatites;    Bone Matrix;    Castor Oil;    Polyurethanes;    Stem Cells;   

DOI  :  10.1590/S0102-86502013000500006

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.

【 授权许可】

CC BY   
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