Animal model of rapid crystalloid infusion in rats

Acta Cirurgica Brasileira

Flavio Stillitano Orgaes²  Fausto Viterbo De Oliveira Neto¹  Flavio Henrique Mendes¹  Renato Florio Yabiku¹ 

[1] ,UNESP Faculty of Medicine of Botucatu Department of Surgery and Orthopedics,Brazil

关键词: Fluid Therapy;    Hemodilution;    Pulmonary Edema;    Models;    Animal;    Rats;   

DOI  :  10.1590/S0102-86502013000400004

来源: SciELO

【 摘 要 】

PURPOSE: To describe an animal model of rapid intravenous infusion with different volumes of crystalloid and discuss the clinical findings. METHODS: Fifty six male Wistar rats were used, divided randomly in seven groups (n = 8). The rats of groups 1 to 6 received lactated Ringer´s solution intravenously, in the rate of 25 ml/min, with different volumes proportional to blood volume (BV). The rats of group 0 were submitted to the same procedure, but did not receive the fluid (control group). The data included respiratory rate, heart rate, saturation of peripheral oxygen (SpO2) in two times (before and after the infusion), and upshots (respiratory arrest and death). Dunnett´s test and ANOVA were used. RESULTS: The clinical signs significantly changed in the 2, 2.5 and 3 fold BV groups. The respiratory arrest was observed in the 1.5, 2, 2.5 and 3 fold BV groups, but death was present only in 2.5 and 3 fold BV groups. CONCLUSIONS: The infusion of crystalloid in the same volume of blood volume did not cause significant variation in respiratory and heart rate, saturation of peripheral oxygen and did not induce respiratory arrest. The infusion of a volume of 3 fold blood volume was lethal to all animals.

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