Animal model for training in sentinel lymph node biopsy of the stomach through combined methods

Acta Cirurgica Brasileira

José Roberto Alves¹  Luiz Roberto Lopes²  Luciana Rodrigues De Meirelles¹  Celso Darío Ramos¹  Tânia Sasasaki¹  Nelson Adami Andreollo¹ 

[1] ,UNICAMP School of Medical Sciences Campinas SP ,Brazil

关键词: Models;    Animal;    Sentinel Lymph Node Biopsy;    Stomach;    Coloring Agents;    Technetium;    Rabbits;    Modelos Animais;    Biópsia de Linfonodo Sentinela;    Estômago;    Corantes;    Tecnécio;    Coelhos;   

DOI  :  10.1590/S0102-86502012001200002

来源: SciELO

【 摘 要 】

PURPOSE: Create and validate a proposed animal model for training in sentinel lymph node biopsy of the stomach. METHODS: In thirty-two rabbits, through a laparotomy, they received a subserosal injection of 0.1 ml of phytate labeled with technetium-99m (0.2 mCi) in the anterior wall of the gastric corpus, followed by 0.2 ml of Blue Patent V® 2.5%, through the same puncture site. Suspicious lymph nodes were searched in vivo at five, ten and 20 minutes, both visually (Blue Patent stained lymph nodes) and with a manual gamma radiation detector (to detect suspected radioactive lymph nodes). After 20 minutes, was performed resection of these for further evaluation of radioactivity (ex vivo) and histological study. RESULTS: Lymph nodes were identified in 30 rabbits (Average of 2.2 lymph nodes per animal). Of the 90 suspected lymph nodes that occurred in the study, 70 cases (77.8%) were histologically confirmed for lymphoid tissue. Of these, the majority were located in the periesophageal region of the gastric fundus. The sample presented a mortality rate of 6.25% and nine complications related to the method, which interfered in the identification of the lymph nodes. CONCLUSION: The animal model for sentinel node biopsy in rabbit stomachs proved to be feasible, with low complexity and reproduced the difficulties encountered for gastric lymph node biopsy in humans, being adequate for surgical training.

【 授权许可】

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