Quantitative histological analysis of the mandibular branch of the facial nerve in rats

Acta Cirurgica Brasileira

Heloisa Juliana Zabeu Rossi Costa¹  Raquel Salomone¹  Ciro Ferreira Da Silva²  Márcio Paulino Costa²  Beatriz Lucchetta Ramos¹  Ricardo Ferreira Bento¹ 

[1] ,USPSao Paulo,Brazil

关键词: Facial Nerve;    Histology;    Rats;    Nervo Facial;    Histologia;    Ratos;   

DOI  :  10.1590/S0102-86502012001100001

来源: SciELO

【 摘 要 】

PURPOSE: To establish a model to quantitative histological analysis of the mandibular branch of the facial nerve in rats. METHODS: Eleven Wistar rats had their right and left mandibular branches of the facial nerve surgically removed and were sacrificed afterwards. Quantitative histological analysis was performed with: a) partial number of axons; b) partial area of the transversal cut of the nerve (9000μm²); c) partial density. The averages of partial density were obtained. The statistical study was established by Wilcoxon test (p=0.05). RESULTS: In relation to density of axons, comparison between sides shows no statistically significant difference (p=0.248; p=0.533). Mean partial density of distal and proximal samples was, respectively, 0.18 ± 0.02 and 0.19 ± 0.02 axons/μm². Comparison between proximal and distal samples shows no statistically significant difference (p=0.859; p=0.182). CONCLUSION: This study has successfully established a model to histological quantitative analysis of the mandibular branch of the facial nerve in rats.

【 授权许可】

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