Effect of N-acetylcysteine in liver ischemia-reperfusion injury after 30% hepatectomy in mice

Acta Cirurgica Brasileira

Edwin Jin Su Lee²  Sonia Maria Da Silva¹  Manuel De Jesus Simões¹  Edna Frasson De Souza Montero¹ 

[1] ,UNIFESP Division of Operative Technique and Experimental Surgery Department of SurgerySao Paulo,Brazil

关键词: Acetylcysteine;    Reperfusion Injury;    Hepatectomy;    Mice;    Acetilcisteína;    Traumatismo por Reperfusão;    Hepatectomia;    Camundongos;   

DOI  :  10.1590/S0102-86502012000400011

来源: SciELO

【 摘 要 】

PURPOSE: Evaluate the effect of N-acetylcysteine in liver remnant after hepatectomy associated to ischemia-reperfusion injury in mice. METHODS: Male adult BALB/c mice, weighing 20-22g were used. Animals were anesthetized with ketamine (70 mg/kg) and xylazine (10 mg/kg); received N-acetylcysteine (150 mg/kg, H-IR-NAC group) or vehicle (H-IR group). Surgical procedures were performed under 10X magnification. Partial hepatectomy (30%) was followed by ischemia-reperfusion injury (30 minutes of ischemia and 60 minutes of reperfusion). Blood sample and liver tissue were removed before animal was euthanized. AST and ALT were evaluated in blood samples and histomorphological analyses were performed in remnant liver. Groups were compared by Mann-Whitney test, and it was considered significant when p<0.05. RESULTS: Biochemical evaluations showed reduced levels of ALT in NAC group (H-IR-NAC=376±127U/l vs H-IR=636±39U/l, p=0.023). AST was similar (p=0.456). H-IR group showed hepatic tissue with preserved architecture, large area of steatosis, vascular congestion and rare mitogenic activity. NAC group showed hepatic tissue with small area of steatosis, vascular congestion and elevated mitogenic activity, evidenced by increased binuclear cells (H-IR-NAC=15.88±0.52 vs H-IR=7.4±0.37, p<0.001). CONCLUSION: N-acetylcysteine promotes enzymatic and morphological protection against hepatectomy and ischemia-reperfusion injury.

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