Transplantation of mouse embryonic stem cell after middle cerebral artery occlusion

Acta Cirurgica Brasileira

Lee Tae-hoon²  Lee Yoon-seok¹ 

[1] ,Namseoul University Department of Emergency Medical Service Chungnam,Korea

关键词: Ischemia;    Embryonic Stem Cells;    Middle Cerebral Artery;    Transplantation;    Mice;    Isquemia;    Células-Tronco Embrionárias;    Artéria Cerebral Média;    Transplante;    Camundongos;   

DOI  :  10.1590/S0102-86502012000400009

来源: SciELO

【 摘 要 】

PURPOSE: Stem cell transplantation has been extensively studied as individual therapies for ischemic stroke. The present investigation is an initial effort to combine these methods to achieve increased therapeutic effects after brain ischemia. Cell transplantation may recover massive neuronal loss by replacing damaged brain cells. METHODS: Undifferentiated mouse embryonic stem (mES) cells were used to induce differentiation in vitro into neuron-like cells with good cell viability for use a graft. In this study, middle cerebral artery occlusion (MCAO) was induced in rats using intra-luminal vascular occlusion, and infused mES cells after MCAO. The animals were examined behaviorally using motor and sensory test with neurological assessment. RESULTS: Motor function of the recipients was gradually improved, whereas little improvement was observed in control rats. This result may suggest that the grafted cells have synaptic connection in the recipient brain. Our study revealed that stem cell transplantation can have a positive effect on behavioral recovery and reduction of infarct size in focal ischemic rats. Consequently after euthanasia, rats were histochemically investigated to explore graft survival with green fluorescent protein (GFP). CONCLUSION: The mouse embryonic stem cells may have advantage for use as a donor source in various neurological disorders including motor dysfunction.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License