Evaluation of the effects of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats undergoing segmental resection and anastomosis of the left colon

Acta Cirurgica Brasileira

Pedro Henrique Alves De Morais¹  Igor Eduardo Caetano De Farias¹  Leonardo De Castro Durães¹  Fabiana Pirani Carneiro²  Paulo Gonçalves De Oliveira²  João Batista De Sousa² 

[1] ,UnB Medical School Brasilia DF ,Brazil

关键词: Abdominal Wall;    Wound Healing;    Pneumoperitoneum;    Artificial;    Tensile Strength;    Rats;    Parede Abdominal;    Cicatrização;    Pneumoperitônio Artificial;    Resistência à Tração;    Ratos;   

DOI  :  10.1590/S0102-86502012000100011

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the influence of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats. METHODS: Eighty rats underwent laparotomy, segmental left colon resection, and anastomosis. The animals were divided into three experimental groups and one control group: EI = pneumoperitoneum for 30 minutes before laparotomy (n=20); EII = pneumoperitoneum for 30 minutes after abdominal closure (n=20); EIII = pneumoperitoneum for 30 minutes before laparotomy and 30 minutes after abdominal closure (n=20); C = control group, without pneumoperitoneum (n=20). In each group, 10 animals were killed 7 days and 10 animals 14 days postoperatively. A segment of the abdominal wall was resected and subjected to tensile strength testing. Another segment of abdominal muscle was used for histopathological analysis; the specimens were fixed in formalin and stained with hematoxylin and eosin. RESULTS: There were no differences in histopathology and tensile strength values among animals in the experimental and control groups 7 or 14 days after surgery. CONCLUSION: Under the present experimental conditions, carbon dioxide pneumoperitoneum did not interfere with abdominal wall wound healing.

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