The effect of progesterone in the prevention of the chemically induced experimental colitis in rats

Acta Cirurgica Brasileira

Oguzhan Karatepe¹  Merih Altiok¹  Muharrem Battal¹  Gulcin Kamali¹  Ahu Kemik¹  Timucin Aydin¹  Servet Karahan¹ 

关键词: Progesterone;    Colitis;    Inflammation;    Colon;    Rats;    Progesterona;    Colite;    Inflamação;    Colo;    Ratos;   

DOI  :  10.1590/S0102-86502012000100005

来源: SciELO

【 摘 要 】

PURPOSE: To study the effects of progesterone on an experimental colitis model. METHODS: Wistar albino rats were treated subcutaneously with 2mg/kg once a day during seven days Colitis was induced by intrarectal administration of 5mg trinitrobenzene sulfonic acid (TNBS). Disease activities, macroscopic and microscopic scores were evaluated. To determine the response provoked by progesterone we measured Colonic malondialdehyde (MDA), TNF alfa, IL-6 and Nitric oxide (NO) levels in addition to the MPO (Myeloperoxidase) and caspase-3 activities. RESULTS: Progesterone ameliorated significantly the macroscopic and microscopic scores. TNBS-induced colitis significantly increased the colonic MDA levels and caspase-3 activities in group 2 in comparison to the control group. The results of the study revealed a decline in MDA, NO, IL6 and TNF-α levels in the colon tissue and in blood due to progesterone therapy in group 3 when compared to the group 2, a significant improvement. Progesterone treatment was associated with decreased MDA, MPO, TNF alfa and caspase-3 activity. CONCLUSION: Progesterone therapy decreased oxidative damage in the colonic mucosa.

【 授权许可】

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