Pancreatic islet allograft in spleen with immunossuppression with cyclosporine. Experimental model in dogs

Acta Cirurgica Brasileira

Jaques Waisberg²  Charles Benjamin Neff¹  Daniel Reis Waisberg¹  Demetrius Germini¹  José Eduardo Gonçalves¹  Arnaldo Zanotto¹  Manlio Basilio Speranzini¹ 

[1] ,Faculty of Medicine of ABC Department of Surgery Santo Andre SP ,Brazil

关键词: Islets of Langerhans Transplantation;    Diabetes Mellitus;    Experimental;    Animal Experimentation;    Dogs;    Transplante das Ilhotas Pancreáticas;    Diabetes Mellitus Experimental;    Experimentação Animal;    Cães;   

DOI  :  10.1590/S0102-86502011000800011

来源: SciELO

【 摘 要 】

PURPOSE: To study the functional behavior of the allograft with immunosuppression of pancreatic islets in the spleen. METHODS: Five groups of 10 Mongrel dogs were used: Group A (control) underwent biochemical tests; Group B underwent total pancreatectomy; Group C underwent total pancreatectomy and pancreatic islet autotransplant in the spleen; Group D underwent pancreatic islet allograft in the spleen without immunosuppressive therapy; Group E underwent pancreatic islet allograft in the spleen and immunosuppression with cyclosporine. All of the animals with grafts received pancreatic islets prepared by the mechanical-enzymatic method - stationary collagenase digestion and purification with dextran discontinuous density gradient, implanted in the spleen. RESULTS: The animals with autotransplant and those with allografts with immunosuppression that became normoglycemic showed altered results of intravenous tolerance glucose (p < 0.001) and peripheral and splenic vein plasmatic insulin levels were significantly lower (p < 0.001) in animals that had allografts with immunosuppression than in those with just autotransplants. CONCLUSIONS: In the animals with immunosupression with cyclosporine subjected to allograft of pancreatic islets prepared with the mechanical-enzymatic preparation method (stationary collagenase digestion and purification with dextran discontinuous density gradient), the production of insulin is decreased and the response to intravenous glucose is altered.

【 授权许可】

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