Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD

Acta Cirurgica Brasileira

Bluma Linkowski Faintuch²  Rodrigo Teodoro¹  Erica Aparecida De Oliveira¹  Eutimio Gustavo Fernández Nuñez¹  Joel Faintuch¹ 

[1] ,University of Sao Paulo IPEN Radiopharmacy CenterSao Paulo SP ,Brazil

关键词: Technetium;    Isotope Labeling;    Radionuclide Imaging;    Ischemia;    Diagnosis;    Rats;    Tecnécio;    Marcação por Isótopo;    Cintilografia;    Isquemia;    Diagnóstico;    Ratos;   

DOI  :  10.1590/S0102-86502011000100011

来源: SciELO

【 摘 要 】

Purpose: Angiogenesis involves many mediators including integrins, and the tripeptide RGD is a target amino acid recognition sequence for many of them. Hindlimb ischemia is a simple and convenient animal model however standardization of the injection procedures in the devascularized and control limb is lacking, thus rendering difficult the interpretation of results. The aim of this investigations was to evaluate neovascularization in a hindlimb murine model by means of 99mTc-HYNIC-ß-Ala-RGD. Methods: 99mTc-HYNIC-RGD analog was prepared using coligands. Ischemia was induced in Wistar rats by double- ligation of the common femoral artery. Radiolabeled RGD was injected after 2h, as well as 1, 3, 5, 7, 10 and 14 days. Uptake was evaluated by planar imaging and biodistribution studies. Results: The highest ratio between ischemia and control was achieved at the 7th day (2.62 ± 0.95), with substantial decrease by the 14th day. For pertechnetate the 7th day ratio was 0.87 ± 0.23. Scintigraphic image confirmed different uptakes. Conclusion: 99mTc-HYNIC-RGD analog concentrated in ischemic tissue by the time of widespread angiogenesis and pertechnetate confirmed reduction in blood flow. In this sense, the protocol can be recommended for ischemic models.

【 授权许可】

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