Use of calcium phosphate cement scaffolds for bone tissue engineering: in vitro study

Acta Cirurgica Brasileira

Taís Somacal Novaes Silva²  Bruno Tochetto Primo¹  Aurelício Novaes Silva Júnior¹  Denise Cantarelli Machado¹  Christian Viezzer¹  Luis Alberto Santos¹ 

[1] ,PUCRS School of Dentistry Department of Oral and Maxillofacial SurgeryPorto Alegre RS ,Brazil

关键词: Biocompatible Materials;    Stem Cells;    Bioengineering;    Bone Cements;    Materiais Biocompatíveis;    Células-Tronco;    Bioengenharia;    Cimentos para ossos;   

DOI  :  10.1590/S0102-86502011000100003

来源: SciELO

【 摘 要 】

Purpose: To evaluate the ability of macroporous tricalcium phosphate cement (CPC) scaffolds to enable the adhesion, proliferation, and differentiation of mesenchymal stem cells derived from human bone marrow. Methods: Cells from the iliac crest of an adult human donor were processed and cultured on macroporous CPC discs. Paraffin spheres sized between 100 and 250µm were used as porogens. Cells were cultured for 5, 10, and 15 days. Next, we assessed cells' behavior and morphology on the biomaterial by scanning electron microscopy. The expression levels of the BGLA and SSP1 genes and the alkaline phosphatase (ALP) activity were quantified by the quantitative real-time polymerase chain reaction technique (QT-PCR) using the fluorophore SYBR GREEN®. Results: QT-PCR detected the expression of the BGLA and SSP1 genes and the ALP activity in the periods of 10 and 15 days of culture. Thus, we found out that there was cell proliferation and differentiation in osteogenic cells. Conclusion: Macroporous CPC, with pore sized between 100 and 250µm and developed using paraffin spheres, enables adhesion, proliferation, and differentiation of mesenchymal stem cells in osteogenic cells and can be used as a scaffold for bone tissue engineering.

【 授权许可】

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