Differential diagnosis in primary and metastatic cutaneous melanoma by FT-Raman spectroscopy

Acta Cirurgica Brasileira

Andrea Fernandes De Oliveira²  Ivan Dunshee De Abranches Oliveira Santos¹  Sidney Bandeira Cartaxo²  Renata Andrade Bitar¹  Mílvia Maria Simões E Silva Enokihara¹  Herculano Da Silva Martinho¹  Airton Abrahão Martin¹  Lydia Masako Ferreira¹ 

[1] ,UNIFESPSao Paulo,Brazil

关键词: Melanoma;    Spectrum Analysis;    Raman;    Diagnosis;    Biopsy;    Melanoma;    Análise Espectral Raman;    Diagnóstico;    Biópsia;   

DOI  :  10.1590/S0102-86502010000500009

来源: SciELO

【 摘 要 】

PURPOSE: To qualify the FT-Raman spectral data of primary and metastatic cutaneous melanoma in order to obtain a differential diagnosis. METHODS: Ten normal human skin samples without any clinical or histopathological alterations, ten cutaneous melanoma fragments, and nine lymph node metastasis samples were used; 105, 140 and 126 spectra were obtained respectively. Each sample was divided into 2 or 3 fragments of approximately 2 mm³ and positioned in the Raman spectrometer sample holder in order to obtain the spectra; a monochrome laser light Nd:YAG at 1064 nm was used to excite the inelastic effect. RESULTS: To differentiate the three histopathological groups according to their characteristics extracted from the spectra, data discriminative analysis was undertaken. Phenylalanine, DNA, and Amide-I spectral variables stood out in the differentiation of the three groups. The percentages of correctly classified groups based on Phenylalanine, DNA, and Amide-I spectral features was 93.1%. CONCLUSION: FT-Raman spectroscopy is capable of differentiating melanoma from its metastasis, as well as from normal skin.

【 授权许可】

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