期刊论文详细信息
Acta Cirurgica Brasileira
A new experimental model for acute hepatic failure in rats
Antonio Roberto Franchi Teixeira2  Marcel Cerqueira Cesar Machado1  Márcia Saldanha Kubrusly1  Nilza Trindade Molan1  Marta Bellodi-privato1  Kátia Regina Leite1  Luiz Augusto Carneiro D'albuquerque1 
[1],USP School of Medicine LIM37-Department of GastroenterologySão Paulo,Brazil
关键词: Liver Failure;    Acute;    Brain Diseases;    Rats;    Falência Hepática Aguda;    Encefalopatias;    Rats;   
DOI  :  10.1590/S0102-86502010000300009
来源: SciELO
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【 摘 要 】
PURPOSE: To develop a reliable surgical model of acute hepatic failure and hyperammonemia in rats that avoids porto-systemic shunt and bile duct ligation, applicable to hepatic encephalopathy research. METHODS: The pedicles of right lateral and caudate lobes were exposed and clamped. One hour later, the animal was reopened, clamps were released and anterior subtotal hepatectomy (resection of median and left lateral lobes) was performed, comprising 75% of liver removal. Four hours after hepatectomy, blood samples and liver tissues were collected from ALF and control groups. RESULTS: Differences between ALF and control groups were significant for ALT, AST, total and direct bilirubin, sodium, potassium, alkaline phosphatasis, gamma-glutamyltransferase and most important, ammonia. Histologically, significant differences were noticed between groups. CONCLUSION: The model is useful for the study of specific aspects of ALF and the development of new therapeutic approaches.
【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

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