Evaluation of placental glycogen storage in mild diabetic rats

Acta Cirurgica Brasileira

Aline Bueno²  Isabela Lovizutto Iessi²  Iracema De Mattos Paranhos Calderon¹  Marilza Vieira Cunha Rudge¹  Carlos Eduardo Meirelles Dos Santos¹  Débora Cristina Damasceno¹ 

[1] ,UNESP Botucatú Medical School Laboratory of Experimental Research of Gynecology and ObstetricsSão Paulo,Brazil

关键词: Diabetes Mellitus;    Insulin;    Pregnancy;    Animal;    Glycogen;    Streptozocin;    Glycemia;    Rats;    Diabetes Mellitus;    Insulina;    Prenhez;    Glicogênio;    Streptozocin;    Glicemia;    Ratos;   

DOI  :  10.1590/S0102-86502010000200002

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the placental glycogen storage and fetal development in the pregnancy of neonatally streptozocin-induced diabetic rats and to establish relation with glycemia and insulin levels. METHODS: At the birth day, 147 female rats were randomly distributed in two experimental groups: 1) Non-diabetic Group (Control, n=45) - received the vehicle; 2) Diabetic Group (STZ, n=102) - received 100 mg streptozocin/kg in neonatal period. At day 0 of pregnancy, adult female rats were included in the control group when presented glycemia below 120 mg/dL and, in the group STZ with glycemia between 120 and 300 mg/dL. At day 21 of pregnancy, blood samples were collected for glycemia and insulin determination, and placentas withdrawn for placental glycogen determination. The newborns (NB) were classified in small (SGA), appropriate (AGA) and large (LGA) for gestational age. RESULTS: Rats STZ presented higher glycemia at days 0 and 14 of pregnancy. At end of pregnancy, rats STZ showed higher proportion of NB SGA and LGA; reduced rate of NB AGA and unaltered glycemia, insulin and placental glycogen determinations. CONCLUSION: Mild diabetes altered the maternal glycemia in the early pregnancy, impairing future fetal development, but it caused no alteration on insulin and placental glycogen determination, confirming that this glycemic intensity was insufficient to change glycogen metabolism.

【 授权许可】

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