Is peritoneal reflection the best anatomical repair landmark in experimental colorectal surgery on rats?

Acta Cirurgica Brasileira

Denise Gonçalves Priolli²  Pamela Lícia Eiras Da Silva¹  Adriane Moro Betini¹  José Aires Pereira¹  Nelson Fontana Margarido¹  Carlos Augusto Real Martinez¹ 

[1] ,Sao Francisco University Medicine School Division of Surgical TechniqueBragança Paulista SP ,Brazil

关键词: Colon;    Collagen;    Anastomosis;    Surgical;    Image Processing;    Computer-Assisted;    Rats;    Cólon;    Colágeno;    Anastomose Cirúrgica;    Processamento de Imagem Assistida por Computador;    Ratos;   

DOI  :  10.1590/S0102-86502009000600011

来源: SciELO

【 摘 要 】

PURPOSE: To validate Peyer's patch as an anatomical repair landmark for colorectal surgery in rats and to measure the collagen content in segments of the colon containing or not containing Peyer's patch. METHODS: The distance between Peyer's patch and the peritoneal reflection was measured in forty-five Wistar rats. The colon and rectum were resected for quantification of collagen content by means of computer-assisted image analysis in regions of the colon with and without Peyer's patch. RESULTS: There was great variation in the distance between Peyer's patch and the peritoneal reflection when the male and female rats were considered as a single group (p=0.04). Comparison between the genders showed that the distance between the patch and the peritoneal reflection was greater in female than in male rats (p=0.001). The colonic segment containing Peyer's patch was observed to have lower tissue collagen content than the segment in which this structure was not present (p=0.02). CONCLUSION: Peyer's patch can be indicated as an anatomical repair landmark, and there is a need to study the healing of colorectal anastomoses in rats based on differing quantities of tissue collagen existing in the colonic wall with or without this structure.

【 授权许可】

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