Experimental splenectomies and malaria in mice

Acta Cirurgica Brasileira

Róbson Miguel De Araújo Negreiros²  Fabiano Hiromichi Makimoto¹  Linda Luciana Oliveira Santana¹  Luís Carlos De Lima Ferreira¹  Gerson Suguiyama Nakajima²  Maria Cristina Dos Santos² 

[1] ,UFAM School of Medicine Surgery Clinical Department Amazonas ,Brazil

关键词: Splenectomy;    Malaria;    Sepsis;    Infection;    Mice;    Esplenectomia;    Malária;    Sepse;    Infecção;    Camundongos;   

DOI  :  10.1590/S0102-86502009000600003

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the importance of spleen in malaric infection in murino model, comparing the parasitemia and the titles of imunoglobulins in the different groups. METHODS: It was used female mice non-isogenic, in inoculated with Plasmodium berghei, cepa ANKA, intraperitoneally. The parasitemia was analyzed in 23rd, 25th, 27th and 32nd day of the experiment, being the stained blood' exam colored by Giemsa. The titles of the total serum immunoglobulins IgM and IgG were analyzed by Dot-ELISA technique, at 6th, 22nd and 32nd day, when the animals were sacrificed. RESULTS: The parasitemia was gradual in all the inoculated groups. In the end of the experiment, the animals with partial parasitemia present superior parasitemia, but next to the non-splenectomized, while the asplenics present difference bigger than the double. The levels of total serum IgM and IgG didin´t have significant changes with the removal partial or total splenic. CONCLUSION: The techniques conservatives in splenic trauma are possible and necessary. The importance of remaining spleen in the clearance of red blood cells parasitized by Plasmodium berghei showed being efficient, in order to avoid serious complications resulting of the malaria in mice.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License