Rat pinealectomy: a modified direct visual approach

Acta Cirurgica Brasileira

Carla Cristina Maganhin²  Ricardo Santos Simões¹  Luiz Fernando Portugal Fuchs¹  Ricardo Martins Oliveira-filho¹  Manuel De Jesus Simões¹  Joaquim Evêncio Neto¹  Edmund Chada Baracat¹  José Maria Soares Júnior¹ 

[1] ,UNIFESP Sao Paulo ,Brazil

关键词: Pineal Gland;    Melatonin;    Genitalia;    Female;    Surgery;    Rats;    Glândula Pineal;    Melatonina;    Genitália Feminina;    Cirurgia;    Ratos;   

DOI  :  10.1590/S0102-86502009000400013

来源: SciELO

【 摘 要 】

PURPOSE: To report a new, direct visual approach for rat pinealectomy. METHODS: Eighty adult female rats (Rattus norvegicus albinus EPM-1 strain) were weighted and anesthetized intraperitoneally with 15 mg/kg xylazine and 30 mg/kg ketamine. The animal was fastened to a dissection table, an incision was made in the skin and the subcutaneous tissue, bringing the lambda into view. The skullcap was opened with a dental drill, bringing the cerebral hemispheres and the superior sagittal sinus into view. The pineal gland, located under the venous sinus, was removed in a single piece using tweezers. Next, the bone fragment was returned to its place and the surgical layers were sutured. RESULTS: This new technique is easy to be done, avoids bleedings and removes only the pineal gland without damage to the remaining encephalon. In addition it makes possible the achievement of a sham surgery, allowing the pineal gland to remain intact. CONCLUSION: The proposed technique intends to facilitate studies aiming to better understanding the complexity and importance of the pineal gland on reproductive and other body systems.

【 授权许可】

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