Association of diabetes and cigarette smoke exposure on the glycemia and liver glycogen of pregnant Wistar rats

Acta Cirurgica Brasileira

Yuri Karen Sinzato²  Paula Helena Ortiz Lima²  Carlos Eduardo Meirelles Dos Santos¹  Kleber Eduardo De Campos²  Marilza Vieira Cunha Rudge¹  Débora Cristina Damasceno² 

[1] ,UNESP Botucatu Medical School Laboratory of Experimental Research in Gynecology and Obstetrics,Brazil

关键词: Diabetes Mellitus;    Experimental;    Pregnancy;    Glycogen;    Tobacco Smoke Pollution;    Glycemia;    Rats;    Diabetes Mellitus Experimental;    Gravidez;    Glicogênio;    Poluição por Fumaça de Tabaco;    Glicemia;    Ratos;   

DOI  :  10.1590/S0102-86502008000600002

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate cigarette smoke exposure and/or diabetes association effects on the glycemia and liver glycogen levels of pregnant Wistar rats. METHODS: 60 adult rats were randomly distributed into (n=10/group): non-diabetic exposed to filtered air (G1); non-diabetic exposed to cigarette smoke only before pregnancy (G2); non-diabetic exposed to cigarette smoke before and during pregnancy (G3); diabetic exposed to filtered air (G4); diabetic exposed to cigarette smoke only before pregnancy (G5), and diabetic exposed to cigarette smoke before and during pregnancy (G6). Glycemia was determined at days 0 and 21 of pregnancy. Liver samples were collected for liver glycogen determinations. RESULTS: At day 21 of pregnancy, glycemia was higher in G5 and G6 compared to G4 group. G2 (2.43±0.43), G3 (3.20±0.49), G4 (2.62±0.34), G5 (2.65±0.27) and G6 groups (1.94±0.35) presented decreased liver glycogen concentrations compared to G1 (4.20±0.18 mg/100mg liver tissue) (p<0.05). G5 and G6 groups presented decreased maternal weight gain and litter weight. CONCLUSIONS: Severe diabetes and cigarette smoke exposure, alone or associated, caused impairment in liver glycogen storage at term pregnancy. Due to the fact that liver glycogen storages were considered determinant for glucose tolerance, it is relevant to point out a rigid clinical glycemic control and to stop smoking so earlier in pregnancy programming.

【 授权许可】

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