Alterations in the intestinal wall due to protein malnutrition in rats: Evaluation of the rupture strength and the tissue's collagen

Acta Cirurgica Brasileira

Victor Nakajima²  Shoiti Kobayasi¹  Luís Eduardo Naresse¹  Celso Vieira De Souza Leite¹  Paulo Roberto Curi¹  Jair Cortez Montovani² 

[1] ,Sao Paulo State University Botucatu Medical School Department of Otorrinolaryngology, Head and Neck Surgery,Brazil

关键词: Protein Malnutrition;    Anastomosis;    Surgical;    Tensile Strength;    Collagen;    Rats;    Desnutrição Proteica;    Anastomose Cirúrgica;    Resistência à Tração;    Colágeno;    Ratos;   

DOI  :  10.1590/S0102-86502008000500008

来源: SciELO

【 摘 要 】

PURPOSE: To study the effect of protein malnutrition on the intestinal wall of rats by evaluating alterations in the rupture force and dosing tissue collagen in the ileum and distal colon. METHODS: One hundred and twenty rats, that had an average weight of 100g, were used. They received water and a standard diet with 20% protein during 7 days for adaptation to the diet itself and to environmental conditions. After that period, the animals were randomly distributed in two groups of 60 rats each: Group 1 - the animals received a control diet with 20% casein for 21 days; Group 2 - hypoprotein diet with 2% casein for 21 days. After the adaptation period, 12 animals of each group were sacrificed at 5 moments: the beginning of experimental period (M0), 4° day (M1), 7° day (M2), 14° day (M3) and 21° day (M4). The diet to the other rats was maintained until the last sacrifice. The following variables were evaluated: body weight, blood albumin rate, tissue's hydroxyproline, hydroxyproline/total protein ratio and rupture strength in the intestinal wall of the ileum and the distal colon. RESULTS: It was observed that the rupture strength in the ileum segment and distal colon was lower in malnourished animals (Group 2); the loss of mechanical resistance was higher in the distal colon segment than in the ileum probably due to the smaller concentration of tissue collagen in the distal colon. CONCLUSION: Protein malnutrition induces the loss of mechanical resistance of the ileum and distal colon and may be associated with a smaller percentage of collagenous tissue formation in the intestinal wall.

【 授权许可】

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