The effect of finasteride on spermatogenesis of Mesocricetus auratus

Acta Cirurgica Brasileira

Dimas José Araújo Vidigal²  Alcino Lázaro Da Silva¹  Luiz Mauro Andrade Da Fonseca¹  Anilton Cesar Vasconcelos¹  Dilermando Fazito De Resende¹  Felipe Eduardo Costa Vidigal¹ 

[1] ,Federal University of Minas Gerais Medicine School ,Brazil

关键词: Mesocricetus;    Finasteride;    Testis;    Spermatogenesis;    Mesocricetus;    Finasterida;    Testículo;    Espermatogênese;   

DOI  :  10.1590/S0102-86502008000300012

来源: SciELO

【 摘 要 】

PURPOSE: To study the effect of finasteride on the spermatogenesis of adult Mesocricetus auratus. METHODS: Twenty adult hamsters were evaluated. The animals were one year-older, and were randomly divided in 2 different groups: control group with ten animals (n=10) and experimental group also with ten animals (n=10). The animals in the experimental group were shot 7.14 ng/mL (0.5mL) of finasteride by 100mg/Kg, subcutaneously in the dorsal region three times per week during 90 days. This dose correspondes to 5mg of the drug used in adult men for the treatment of benign prostatic hyperplasia (BPH). After three months, the animals were anesthetized through association of 200mg/kg ketamine chloridrate and 2.5 mg/kg of diazepan and were dead through hypovolemia.. The testis removed along with the whole genitourinary apparel were fixed with 10% formalin and submitted to histological analisys by optical microscopy. The hematoxilin-eosin (HE) method was used to stain the slides. RESULTS: The mean weight of animals in the control group before death was 129.0±18.8gr. The mean weight of animals in experimental group was 145.0±15.25gr. The mean age of animals in control group before death was 15.2±1.13 months. The mean age of animals in experimental group before death was 17.16±0.82 months. The mean difference in weight between both groups was not statistical significant (p=0.0514). The totality of animals in control group (100%) presented no tubular alterations and showed no disturbancy in the spermatogenesis stages. Four animals (40%) in the experimental group showed hypotrophy of the seminiferous tubules and six (60%) showed normal spermatogenesis, however reduced compared to control group. There was statiscally significant difference (p=0.043) between the control and experimental group related to testicular alterations. CONCLUSION: The animals that were administered finasteride showed significant tubules atrophy and spermatogenesis reduction compared to control group.

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