Acta Cirurgica Brasileira | |
Experimental rat lung tumor model with intrabronchial tumor cell implantation | |
Antero Gomes Neto2  Antônio Felipe Leite Simão2  Samuel De Paula Miranda2  Lívia Talita Cajaseiras Mourão2  Nilfácio Prado Bezerra2  Paulo Roberto Carvalho De Almeida1  Ronaldo De Albuquerque Ribeiro1  | |
[1] ,Federal University of Ceará,Brazil | |
关键词: Lung neoplasms; Walker-256 carcinoma; rats; Neoplasias Pulmonares; Carcinoma 256 de Walker; Ratos; | |
DOI : 10.1590/S0102-86502008000100014 | |
来源: SciELO | |
【 摘 要 】
PURPOSE: The objective of this study was to develop a rat lung tumor model for anticancer drug testing. METHODS: Sixty-two female Wistar rats weighing 208 ± 20 g were anesthetized intraperitoneally with 2.5% tribromoethanol (1 ml/100 g live weight), tracheotomized and intubated with an ultrafine catheter for inoculation with Walker's tumor cells. In the first step of the experiment, a technique was established for intrabronchial implantation of 10(5) to 5×10(5) tumor cells, and the tumor take rate was determined. The second stage consisted of determining tumor volume, correlating findings from high-resolution computed tomography (HRCT) with findings from necropsia and determining time of survival. RESULTS: The tumor take rate was 94.7% for implants with 4×10(5) tumor cells, HRCT and necropsia findings matched closely (r=0.953; p<0.0001), the median time of survival was 11 days, and surgical mortality was 4.8%. CONCLUSION: The present rat lung tumor model was shown to be feasible: the take rate was high, surgical mortality was negligible and the procedure was simple to perform and easily reproduced. HRCT was found to be a highly accurate tool for tumor diagnosis, localization and measurement and may be recommended for monitoring tumor growth in this model.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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