Differential astroglial responses in the spinal cord of rats submitted to a sciatic nerve double crush treated with local injection of cultured Schwann cell suspension or lesioned spinal cord extract: implications on cell therapy for nerve repair

Acta Cirurgica Brasileira

João Gabriel Martins Dallo²  Bernardo Vergara Reichert²  José Benedito Ramos Valladão Júnior²  Camila Silva¹  Bianca Aparecida De Luca¹  Beatriz De Freitas Azevedo Levy¹  Gerson Chadi¹ 

[1],USP School of Medicine São Paulo,Brazil

关键词: Sciatic nerve lesion;    Astrocyte;    Neuronal protection;    Neuroplasticity;    Neuroregeneration;    Pain;    Review;    Lesão do nervo ciático;    Astrócito;    Proteção neuronal;    Neuroplasticidade;    Neurorregeneração;    Dor;    Revisão;   

DOI  :  10.1590/S0102-86502007000600013

来源: SciELO

【 摘 要 】

PURPOSE: Reactive astrocytes are implicated in several mechanisms after central or peripheral nervous system lesion, including neuroprotection, neuronal sprouting, neurotransmission and neuropathic pain. Schwann cells (SC), a peripheral glia, also react after nerve lesion favoring wound/repair, fiber outgrowth and neuronal regeneration. We investigated herein whether cell therapy for repair of lesioned sciatic nerve may change the pattern of astroglial activation in the spinal cord ventral or dorsal horn of the rat. METHODS: Injections of a cultured SC suspension or a lesioned spinal cord homogenized extract were made in a reservoir promoted by a contiguous double crush of the rat sciatic nerve. Local injection of phosphate buffered saline (PBS) served as control. One week later, rats were euthanized and spinal cord astrocytes were labeled by immunohistochemistry and quantified by means of quantitative image analysis. RESULTS: In the ipsilateral ventral horn, slight astroglial activations were seen after PBS or SC injections, however, a substantial activation was achieved after cord extract injection in the sciatic nerve reservoir. Moreover, SC suspension and cord extract injections were able to promote astroglial reaction in the spinal cord dorsal horn bilaterally. Conclusion: Spinal cord astrocytes react according to repair processes of axotomized nerve, which may influence the functional outcome. The event should be considered during the neurosurgery strategies.

【 授权许可】

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