Pancreatic intraepithelial neoplasia and ductal adenocarcinoma induced by DMBA in mice: effects of alcohol and caffeine

Acta Cirurgica Brasileira

Luiz Roberto Wendt²  Alessandro Bersch Osvaldt²  Vivian Pierre Bersch¹  Rita De Cássia Schumacher¹  Maria Isabel Albano Edelweiss¹  Luiz Rohde¹ 

[1] ,UFRGS Surgery Post-Graduation Program ,Brazil

关键词: Caffeine;    9;    10-Dimethyl-1;    2-benzanthracene;    Adenocarcinoma;    Pancreas;    Mice;    Cafeína;    9;    10-Dimetil-1;    2-benzantraceno;    Adenocarcinoma;    Pâncreas;    Camundongos;   

DOI  :  10.1590/S0102-86502007000300008

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the effects of alcohol and caffeine in a pancreatic carcinogenesis mouse model induced by 7,12-dimethylbenzantracene (DMBA), according to the PanIN classification system. METHODS: 120 male, Mus musculus, CF-1 mice were divided into four groups. Animals received either water or caffeine or alcohol or alcohol + caffeine in their drinking water. In all animals, 1 mg of DMBA was implanted into the head of the pancreas. After 30 days, euthanasia was performed; excised pancreata were then fixed in formalin, stained with hematoxylin-eosin and categorized as follows: normal ducts, reactive hyperplasia, PanIN-1A, PanIN-1B, PanIN-2, PanIN-3 or adenocarcinoma. RESULTS: PanIN lesions were verified in all groups. Adenocarcinoma was detected in 15% of animals in the caffeine group, 16.6% in the water group, 23.8% in the alcohol + caffeine group and 52.9% in the alcohol group (P<0.05). CONCLUSIONS: The experimental pancreatic carcinogenesis mouse model using DMBA effectively induces PanIN lesions and pancreatic adenocarcinoma. This study verified the association between alcohol use and pancreatic adenocarcinoma; caffeine did not present the same effect.

【 授权许可】

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