Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique

Acta Cirurgica Brasileira

Eduardo Crema²  Ana Márcia Werneck¹  Aiodair Martins Júnior²  Lara Beatriz Prata Ribeiro¹  Tatiana Silva De Lima¹  Alex Augusto Silva² 

[1] ,UFTM Department of Digestive Surgery ,Brazil

关键词: Cell Separation;    Cell Transplantation;    Edetic Acid;    Separação Celular;    Transplante de Células;    Ácido Edético;   

DOI  :  10.1590/S0102-86502007000100010

来源: SciELO

【 摘 要 】

PURPOSE: To compare the viability of human hepatocytes dissociated by the ethylenediaminetetraacetic acid and collagenase techniques. METHODS: Hepatocytes were prepared by dissociation of liver fragments obtained from hepatectomies performed for therapeutic purposes at the Service of Digestive Tract Surgery, Federal University of Triângulo Mineiro. RESULTS: During the first 4 days of the experiment, 70% of the cells presented birefringent membranes and were not stained with 2% erythrosine, and were therefore considered to be viable. During the first 3 days, hepatocyte viability was on average 71% in the EDTA group and 76% in the collagenase group, with no significant difference between groups. No significant difference was observed between groups at any time. The secretion of albumin by the cultured hepatocytes was preserved up to the seventh day. Mean albumin secretion during the first 3 days was 50 µg/ml in the two groups and a reduction of albumin production was observed from the fourth to the seventh day. Again, no significant difference was observed between groups at any time. CONCLUSION: Cell viability and preservation of albumin secretion by hepatocytes are similar for the EDTA and collagenase techniques.

【 授权许可】

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