Usefulness of argyrophilic nucleolar organizer regions in detection of lung cells alterations after benzo[a]pyrene instillation

Acta Cirurgica Brasileira

Baldomero Antonio Kato Da Silva²  Iandara Schettert Silva¹  Daniel Martins Pereira¹  Ricardo Dutra Aydos¹  Paulo De Tarso Camillo De Carvalho¹ 

[1] ,UnB,Brazil

关键词: Benzo[a]pyrene;    Biological Markers;    Nucleolus Organizer Regions;    Benzo[a]pireno;    Marcadores Biológicos;    Região Organizadora do Nucléolo;   

DOI  :  10.1590/S0102-86502006001000009

来源: SciELO

【 摘 要 】

PURPOSE: To verify the relationship between AgNOR expression and lung tissues changes of Wistar rats after pulmonary instillation of benzo[a]pyrene (B[a]P). METHODS: Male Rattus norvegicus albinus,Wistar lineage were given a single intrapulmonary instillation of B[a]P at doses of 10 and 20 mg/kg in a volume of approximately 0,3 ml. After 7 and 21 days the rats were killed and the lung slices submitted to a histological technique of AgNOR. AgNOR dots were quantified and the result analyzed by statistical tests; p <= 0,05 was considered significant. RESULTS: The mean values of AgNOR dots for the experimental groups 10/7 (1,51±0,86) and 10/21 (1,84±0,13) were statistically different (p = 0,009). Among the groups 20/7 (1,63±0,11) and 20/21 (2,48±0,28) was observed statistically significant difference (p = 0,003). CONCLUSION: The AgNOR technique can be useful in identification of cells changes induced by B[a]P.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License