Photodynamic inactivation of in vitro bacterial cultures from pressure ulcers

Acta Cirurgica Brasileira

Paulo De Tarso Camillo De Carvalho²  Ana Paula Da Costa Marques²  Felipe Abdalla Dos Reis²  Ana Carulina Guimarães Belchior²  Iandara Schettert Silva¹  Carlos Alexandre Habitante¹  Daniela Aparecida Sussai¹ 

[1] ,UNIDERP Department of Physiotherapy ,Brazil

关键词: Photosensitizing Agents;    Anti-Bacterial Agents;    Skin Ulcers;    Agentes Fotossensibilizantes;    Agentes Antibacterianos;    Úlceras Cutâneas;   

DOI  :  10.1590/S0102-86502006001000008

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate in vitro the antibacterial effect of diode laser light of wavelength 650 nm, in association with the photosensitive substance toluidine blue, on the bacteria in infected skin ulcers. METHODS: Samples were collected by means of swabs containing a medium for transporting infected material from skin ulcers. The material was inoculated into culturing medium containing azide blood agar for the growth of Gram-positive bacteria, and MacConkey agar for Gram-negative bacteria, and incubated for 48 hours. The results obtained from counting the colony-forming units were correlated and subjected to statistical analysis, adopting the significance level of p > or =0.05. RESULTS: From analysis of variance (ANOVA), the result for the general mean was p = 0.0215. Using the t test with post-hoc test, the result for TBO vs. Control was p = 0.0186, and for TBO + Laser vs. Control it was p = 0.0039. CONCLUSION: There was a significant reduction in colony-forming units when the cultures were subjected to photodynamic therapy.

【 授权许可】

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