Effects of the combined use of glutamine and growth hormone in the intestinal adaptation after massive resection of the small bowel in rats

Acta Cirurgica Brasileira

Joaquim M. Spadoni²  José Eduardo De Aguilar-nascimento¹  Maria H.g. Gomes Da Silva¹  Bruno Spadoni-neto²  Priscila Arruda Thulio F. Batista Da Costa¹  Denise Maria T. Aléssio¹ 

[1] ,University of Cuiabá Department of Surgery

关键词: Glutamine;    Growth hormone;    Intestinal mucosa;    Short bowel syndrome;    DNA;    Rats;    Glutamina;    Hormônio do crescimento;    Mucosa intestinal;    Síndrome do intestino curto;    DNA;    Ratos;   

DOI  :  10.1590/S0102-86502005000500008

来源: SciELO

【 摘 要 】

PURPOSE: The aim of this study was to investigate the effects of the combined use of glutamine (GL) and growth hormone (GH) in the intestine of rats submitted to 80% small bowel resection. METHODS: [24] Twenty four Wistar rats were randomized to receive either a standard rat chow - control group (CG, n=12) or the same diet added to 4% glutamine - GL-GH group (n=12) after 80% enterectomy. The latter group received subcutaneously 0,6UI/day of GH. Groups of six rats in each group were killed on the 5th and 14th days. The following variables were studied: body weight, mucosal weight, histomorphometry and DNA content in the resected specimen and in the adapted intestines after necropsy. RESULTS: All animals lost weight stabilizing after the 5th PO day in both groups. There was not any statistical difference in the mucosal weight associated to groups and dates. However, ileal mucosal weight decreased from basal to final results when compared to jejunal mucosa (p= 0.02). The DNA content increased from the initial to the final results (p <0.001) in both groups, though, this increase was greater in GL-GH animals (CG = 0.53 [95% CI, 0.44-0.62] g/cm-1 vs. GL-GH= 0.85 [95%CI, 0.76-0.94] g/cm-1; p<0.01), especially at the 14th day. Ileal DNA content was significantly greater than jejunal (p=0.01). There was a significant increase in the intestinal wall width and crypt depth in the control group (p<0.01). CONCLUSION: Gut adaptation after massive resection is improved with the combined use of glutamine and GH.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License