Chimerism induction by nonmyeloablactive preconditioning and bone marrow infusion in rat small bowel transplantation

Acta Cirurgica Brasileira

Alexandre Bakonyi Neto²  Mariana Behro¹  Phillip Ruiz¹  Evangelos P. Misiakos¹  Joshua Miller¹  Bonifacio K. Takegawa²  Camilo Ricordi¹  Andreas G. Tzakis¹ 

[1] ,UNESP Faculty of Medicine Department of Surgery

关键词: Intestinal transplant;    Bone marrow infusion;    Irradiation;    Transplante intestinal;    Infusão de medula óssea;    Irradiação;   

DOI  :  10.1590/S0102-86502003000100003

来源: SciELO

【 摘 要 】

In our previous work we demonstrated that the use of donor specific bone marrow infusions ( DSBMI ) after small bowel transplantation did not improve the graft survival after a short course of immunossupression. PURPOSE: In the current study, we evaluated whether recipient preconditioning with different regimens of radiation combined with DSBMI may enhance small bowel allograft survival with minimum recipient morbidity. METHODS: Heterotopic small bowel transplantation (SBTx) was performed with Lewis rats as recipients and DA rats as donors, which were immunossupressed with a short course of tacrolimus (FK 506 ) at 1mg/Kg/day for 5 days and distributed in 4 groups: group 1 (n= 4) without both irradiation and DSBMI; Groups 2 (n= 6), 3 (n= 9) and 4 (n= 6) received 100 x 10(6) DSBM cells at the time of the transplant. Groups 3 and 4 were irradiated with 250 and 400 rd respectively. Animals were examined daily for clinical signs of rejection or GVHD. Blood samples were taken weekly for chimeric studies by FC and intestinal biopsies were performed every 2 weeks. RESULTS: Animals in G1 and G2 had minimal rejection at day 15 after SBTx while GVHD was clinically and histologically characterized in G 3 and G 4. Total chimerism and T-cell chimerism was higher in irradiated groups when compared to non-irradiated groups. With exception of G1 and 2 where rejection was the cause of death, all animals in G3 and 4 died of GVHD. CONCLUSION:We concluded that low cytoreductive of irradiation can successfully decrease the graft rejection but not prevent the occurrence of GVHD.

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