Led enhancement in mitochondrial oxidative phosphorylation for hepatectomized rats

Acta Cirurgica Brasileira

Orlando De Castro-e-silva Jr²  Tiago Castro-e-silva¹  Fernando Silva Ramalho²  Leandra Naira Zambelli Ramalho¹  Sergio Zucoloto¹  Luis Gustavo Marcassa¹  Vanderlei Salvador Bagnato¹ 

[1] ,USP FMRP Department of Surgery and Anatomy

关键词: Liver;    mitochondrial function;    phosphorylation index;    partial hepatectomy;   

DOI  :  10.1590/S0102-86502002000900019

来源: SciELO

【 摘 要 】

Recently, the LED (light emitting diode) developed by the Optics Group of IFSC-USP has been used instead of laser for the treatment of skin tumors by the PDT (Photodinamic Therapy) because of its low operational cost compared to the use of a laser. In this paper we investigate the effect of LED light on oxidative phosphorylation during liver regeneration after partial hepatectomy. Twenty-four male Wistar rats (250 g) were kept in identical housing units on a 12-hour light/12 hour dark cycle. The LED 10 group was exposed to LED at 638 nm (10 J/cm² for 3 minutes). Seventy percent partial hepatectomy was performed in the LED 10 and HPC (Partial Hepatectomy-Control). A sham-operated group (C) was used for control. Twenty four hours after the procedure, LED 10, HPC and control animals were sacrificed. Samples of liver tissue were used for the mitochondrial respiration assay. Statistical comparisons of the groups were performed by analysis of variance (ANOVA), followed by the Bonferroni post-test. Probability values less than 0.05 were considered to be statistically significant. the phosphorylation index (FI) for the LED 10 group was higher than that for the HPC group and for the sham group (p<005). The FI for the HPC group was higher than that for the sham group (p<005). The values of the ADP:O ratio for the three groups, which did not differ significantly from one another (p > 0.05). In the present study we noted an effective interaction between LED light and hepatic mitochondria, with an increased phosphorylation rate for the latter.

【 授权许可】

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