Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody

Revista Brasileira de Cirurgia Cardiovascular

Chun-li Song¹  Qian Li¹  Yun-peng Yu¹  Guan Wang¹  Jin-peng Wang¹  Yang Lu¹  Ji-chang Zhang¹  Hong-ying Diao¹  Jian-gen Liu¹  Yi-hang Liu¹  Jia Liu¹  Ying Li¹  Dan Cai¹  Bin Liu¹ 

关键词: Coronary Artery Disease;    Drug-Eluting Stents;    Coronary Restenosis;    Vascular Endothelial Growth Factor;    Antigens;    CD34;    Doença Arterial Coronariana;    Stents Farmacológicos;    Reestenose Coronária;    Fator de Crescimento Endotelial Vascular;    Antígenos CD 34;   

DOI  :  10.5935/1678-9741.20150016

来源: SciELO

【 摘 要 】

AbstractIntroduction:Intravascular coronary stenting has been used in the treatment of coronary artery disease (CAD), with a major limitation of in-stent restenosis (ISR). The 316 stainless steel has been widely used for coronary stents. In this study, we developed a novel coating method to reduce ISR by simultaneously coating vascular endothelial growth factor (VEGF) and anti-CD34 antibody on 316L stainless steel.Methods:Round 316L stainless steel sheets in the D-H group were polymerized with compounds generated from condensation reaction of dopamine and heparin using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). Sixteen sheets from the D-H group were further immersed into 1ug/ml VEGF165 and 3mg/ml heparin sodium one after another for 10 times, and named as the D-(H-V)10 group. Eight sheets from the D-(H-V)10 group were coated with anti-CD34 antibody and termed as the D-(H-V)10-A group. Immunofluorescence assay and ELISA were used to evaluate whether the 316L stainless steel disks were successfully coated with VEGF and anti-CD34 antibody.Results:The results of immunofluorescence assay and ELISA showed that VEGF could be detected in the D-(H-V)10 and D-(H-V)10-A group, suggesting the steel sheets were successfully covered with VEGF. Anti-CD34 antibody could only be observed in the D-(H-V)10-A group, which was the only group coated with CD34 antibody. Both results suggested that the 316L stainless steel sheets were successfully coated with VEGF and anti-CD34 antibody.Conclusion:Our study developed a method to simultaneously coat VEGF and anti-CD34 antibody to stainless metal steel. This research serves as a fundamental role for a novel coating strategy.

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