期刊论文详细信息
Revista Brasileira de Cirurgia Cardiovascular
Comparison of two surgical techniques for creating an acute myocardial infarct in rats
Luiz Guilherme Achcar Capriglione1  Fabiane Barchiki1  Gabriel Sales Ottoboni1  Nelson Itiro Miyague1  Paula Hansen Suss1  Carmen Lúcia Kuniyoshi Rebelatto1  Cláudia Turra Pimpão1  Alexandra Cristina Senegaglia1  Paulo Roberto Brofman1 
关键词: Myocardial infarction;    Echocardiography;    Models;    Cardiovascular;    Infarto do miocárdio;    Ecocardiografia;    Modelos Cardiovasculares;   
DOI  :  10.5935/1678-9741.20140075
来源: SciELO
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【 摘 要 】

Objective: To perform a comparative assessment of two surgical techniques that are used creating an acute myocardial infarc by occluding the left anterior descending coronary artery in order to generate rats with a left ventricular ejection fraction of less than 40%. Methods: The study was completely randomized and comprised 89 halothane-anaesthetised rats, which were divided into three groups. The control group (SHAM) comprised fourteen rats, whose left anterior descending coronary artery was not occluded. Group 1 (G1): comprised by 35 endotracheally intubated and mechanically ventilated rats, whose left anterior descending coronary artery was occluded. Group 2 (G2): comprised 40 rats being manually ventilated using a nasal respirator whose left anterior descending coronary artery was occluded. Other differences between the two techniques include the method of performing the thoracotomy and removing the pericardium in order to expose the heart, and the use of different methods and suture types for closing the thorax. Seven days after surgery, the cardiac function of all surviving rats was determined by echocardiography. Results: No rats SHAM group had progressed to death or had left ventricular ejection fraction less than 40%. Nine of the 16 surviving G1 rats (56.3%) and six of the 20 surviving G2 rats (30%) had a left ventricular ejection fraction of less than 40%. Conclusion: The results indicate a tendency of the technique used in G1 to be better than in G2. This improvement is probably due to the greater duration of the open thorax, which reduces the pressure over time from the surgeon, allowing occlusion of left anterior descending coronary artery with higher accuracy.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

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