Transforming growth factor-β/Smad signaling function in the aortopathies

Revista Brasileira de Cirurgia Cardiovascular

Shi-min Yuan²  Jun Wang¹  Xiao-nan Hu¹  De-min Li¹  Hua Jing¹ 

[1] ,Affiliated Hospital of Taishan Medical College Department of Cardiothoracic Surgery Taian Shandong Province ,People's Republic of China

关键词: Aorta;    Aorta;    Thoracic;    Smad Proteins;    Transforming Growth Factor beta1;    Aorta;    Aorta Torácica;    Fatores Transformadores de Crescimento;    Proteínas Smad;   

DOI  :  10.5935/1678-9741.20110014

来源: SciELO

【 摘 要 】

OBJECTIVES: Transforming growth factor (TGF)-β/Smad signaling pathway in aortic dissection patients and normal subjects has not been previously described. The present study was designed to evaluate the TGF-β/Smad signaling expressions in the patients with acute type A aortic dissection in comparison with those in the patients with thoracic aortic aneurysm and with coronary artery disease, and (or) the healthy subjects. METHODS: Consecutive surgical patients for acute type A aortic dissection (20 patients), aortic aneurysm (nine patients) or coronary artery disease (20 patients) were selected into this study. Blood samples (4 ml) were obtained from the right radial arterial indwelling catheter after systemic heparinization prior to the start of cardiopulmonary bypass in the operating room. Twenty-one young healthy volunteers without underlying health issues who donated forearm venous blood samples (4 ml) were taken as control. The surgical specimens of the aortic tissues were obtained immediately after they were severed during the operations of the replacement of the aorta in the patients with aortic dissection or aortic aneurysm. In patients receiving coronary artery bypass grafting, the tiny aortic tissues were taken when the punch holes of the proximal anastomosis on the anterior wall of the ascending aorta were made. The aortic tissues were for RNA, protein, or supernatant preparations until detection of TGF-β1 mRNA by quantitative real-time reverse transcription polymerase chain reaction, of TGF-β1, TGF-β receptor I, Smad2/3, Smad4 and Smad7 by Western blot, and of TGF-β1 by enzyme-linked immunosorbent assay, respectively.

【 授权许可】

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