Triterpenes from the Protium heptaphyllum resin - chemical composition and cytotoxicity

Revista Brasileira de Farmacognosia

Ewelyne M. Lima¹  Andrews M. Nascimento¹  Dominik Lenz¹  Rodrigo Scherer¹  Silvana S. Meyrelles¹  Giovanna A.p. Boëchat¹  Tadeu U. Andrade¹  Denise C. Endringer¹ 

关键词: α-Amyrin;    β-Amyrin;    Almíscar;    Angiotensin converting;    enzyme activity;    Caspase-3;    TNF-α;   

DOI  :  10.1016/j.bjp.2014.06.003

来源: SciELO

【 摘 要 】

Protium heptaphyllum (Aubl) Marchand, Burseraceae, is popularly used as an analgesic and anti-inflammatory agent. However, the cellular mechanism of action remains unknown. This study aims to evaluate the chemical composition of P. heptaphyllum resin and cytotoxicity on a breast cancer cell line (MCF-7). The chemical composition of the resin was determined by Gas Chromatography coupled to a Mass Spectrometer. The cytotoxicity was evaluated using an MTT assay. Annexin V-FITC, caspase-3, Angiotensin Converting Enzyme activity and Tumor Necrosis Factor alpha (TNF- α) assays were performed to evaluate apoptosis and inflammatory events. The resin consisted of triterpenes, such as α- and β-amyrin. Cytotoxicity was only observed in fractions enriched with α- and β-amyrin. The resin and fractions elicited antiproliferative activity, increased activity of caspase-3 and ACE, and a decrease in the TNF-α level. Altogether, the resin and fractions enriched with α- and β-amyrin promoted cytotoxicity and apoptosis.

【 授权许可】

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