Box-Behnken design to study the bergenin content and antioxidant activity of Endopleura uchi bark extracts obtained by dynamic maceration

Revista Brasileira de Farmacognosia

Luciana A. Tacon¹  Luis A. P. Freitas¹ 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto ,Brazil

关键词: design of experiments;    DPPH;    hydroalcoholic;    polyphenol;   

DOI  :  10.1590/S0102-695X2012005000134

来源: SciELO

【 摘 要 】

Brazil has one of the world's largest biodiversity in flora and a plant that has attracted attention is the Endopleura uchi (Huber) Cuatrec., Humiriaceae, which is native of the Brazilian Amazon. Among the many popular uses, this species is utilized in the treatment of woman's genito urinary tract affections and also as anti-inflammatory. It is believed that their actions derive from the major constituent, bergenin. The objective of this work was to study the Endopleura uchi barks extraction using the dynamic maceration method and the effects of the extraction time, drug to solvent ratio and temperature. A Box-Behnken design was applied to study the influence of these factors and the respective response surfaces. The extract characterization was made by determination of its antioxidant activity by DPPH; total polyphenol content and bergenin content. In general, the extracts showed good antioxidant activity, with the IC50 ranging from 4.02 to 5.87 µg/mL. The polyphenol content ranged from 31.89 to 47.82%. High levels of chemical markers are observed in all extracts, with average bergenin content of 35.58%. The result showed that the multivariate study of extraction is key step in the development and standardization of extracts Endopleura uchi.

【 授权许可】

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