Chemical composition and cytotoxicity activity of the essential oil of Pterodon emarginatus

Revista Brasileira de Farmacognosia

Rafael C. Dutra²  Frederico Pittella²  Dalton Dittz¹  Rodrigo Marcon¹  Daniel S. Pimenta¹  Miriam T. P. Lopes¹  Nádia R. B. Raposo² 

[1] ,Universidade Federal de Juiz de Fora Núcleo de Identificação e Quantificação Analítica ,Brazil

关键词: trans-caryophyllene;    cytotoxic activity;    β-elemene;    essential oil;    Pterodon emarginatus;    Leguminosae;   

DOI  :  10.1590/S0102-695X2012005000042

来源: SciELO

【 摘 要 】

Pterodon emarginatus Vogel, Fabaceae, is a native aromatic tree distributed by central region of Brazil. Hydroalcoholic infusions of the seeds are used in folk medicine for their anti-rheumatic and anti-inflammatory properties. The objective of this work was identified the chemical components and verify the cytotoxic effect of the essential oil (EO) from P. emarginatus seeds. Thus, the EO of P. emarginatus seeds was analyzed by GC/MS analysis followed by brine shrimp lethality test and cytotoxic activity against tumor cell lines and human peripheral mononuclear blood cells (PBMC). The cancer cell lines tested were C6 (rat glioma), MeWo (human melanoma), CT26.WT (mouse colon carcinoma), MDA (human breast cancer), A549 (human lung carcinoma), B16-F1 (mouse melanoma), CHO-K1 (hamster ovary cell) and BHK-21 (hamster kidney fibroblast). Eleven compounds were identified by GC and CG/MS analyses. The main compounds with concentrations higher than 5% were β-elemene (15.3%), trans-caryophyllene (35.9%), α-humulene (6.8%), germacrene-D (9.8%), bicyclo germacrene (5.5%) and spathulenol (5.9%). The EO of P. emarginatus seeds showed toxicity to Artemia salina (LC50 1.63 µg/mL) and was active against all the cell lines tested. The potent cytotoxic activity had IC50 values ranging from 24.9 to 47 µg/mL. However, EO (1-100 µg/mL) had less cytotoxicity in PBMCs isolated from a healthy subject. In summary, the present study showed the potential antiproliferative of the EO of P. emarginatus seeds.

【 授权许可】

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