The antigenotoxic activity of latex from Himatanthus articulatus

Revista Brasileira de Farmacognosia

Sebastião De O. Rebouças¹  Juliana Da Silva¹  Aline A. Groff¹  Emilene A. Nunes¹  Martus Ianistcki¹  Alexandre De B. F. Ferraz¹ 

[1] ,Universidade Luterana do Brasil Programa de Pós-graduação em Genética e Toxicologia Aplicada

关键词: antigenotoxic;    comet assay;    genotoxic;    Himatanthus articulatus;    latex;   

DOI  :  10.1590/S0102-695X2011005000216

来源: SciELO

【 摘 要 】

Himatanthus articulatus (Vahl) Woodson (Apocynaceae) is a native plant to the Amazon popularly used to treat ulcers, tumors, inflammations, cancer, syphilis and malaria. The aim of the present study was to investigate the in vivo genotoxic/antigenotoxic and mutagenic potential of this plant, using the comet and the micronucleus assays in mice. Female and male adult mice were treated with different doses of H. articulatus latex by gavage for two consecutive days. For the experiments, the latex was serially diluted with water to 1:2 (D1); 1:4 (D½) and 1:8 (D¼) and administered to the animals. The blood slides were exposed to hydrogen peroxide (ex vivo) to evaluate antigenotoxic effect. Under the experimental conditions used in this study, the latex of H. articulatus did not increase the frequency of DNA damage as measured by the comet assay and micronucleus test in treated mice, indicating a non-genotoxic and non-mutagenic activity. In relation to the antigenotoxicity, latex exerted protective effect against DNA damage induced by hydrogen peroxide. Therefore, our results add new information about the antigenotoxic potential of H. articulatus latex, which is popularly used in the Amazon to treat different pathologies.

【 授权许可】

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