Essential oil composition and isolation of freeradical-scavenging phenolic glycosides from the aerial parts of Ajuga chamaepitys growing in Iran

Revista Brasileira de Farmacognosia

Abbas Delazar²  Mohammad-reza Delnavazi¹  Narges Yassa¹  Shiva Parkhideh²  Nima Delazar²  Lutfun Nahar¹  Satyajit D. Sarker¹ 

[1] ,Tabriz University of Medical Sciences School of Pharmacy and Drug Applied Research Center ,Iran

关键词: acteoside;    Ajuga chamaepitys;    2;    2-diphenyl-1-picryl-hydrazyl (DPPH);    essential oil;    flavone glycoside;    free-radical-scavenger;    GC-MS;    GC-FID;    Lamiaceae;   

DOI  :  10.1590/S0102-695X2011005000215

来源: SciELO

【 摘 要 】

From the methanolic extract of the aerial parts of Ajuga chamaepitys (L.) Schreb., Lamiaceae, one of the Iranian medicinal plants, the phenylethanoid glycoside, acteoside, and two flavone glycosides, chrysoeriol 7-O-glucopyranoside (3'-methoxy-luteolin 7-O-glucopyranoside) and apigenin 7-O-rhamnopyranoside, were isolated by a combination of solid-phase extraction (SPE) and preparative reversed-phase high-performance liquid chromatography (prep-RP-HPLC) methods. Structures of the isolated compounds were elucidated by spectroscopic means. The free-radical-scavenging properties of the extracts, fractions and isolated compounds were determined by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. While among the extracts, the MeOH extract showed the highest level of free-radical-scavenging activity (RC50 1.15 × 10-1 mg/mL), chrysoeriol 7-O-glucopyranoside was the most active (RC50 3.00 × 10-3 mg/mL) among the isolated compounds. The GC-MS and the GC-FID analyses revealed α-pinene (23.66%), β-pinene (9.33%), 1-octen-3-ol (9.72%), β-phellandrene (8.70%) and germacrene-D (7.92%) as the major components of the essential oils derived from the aerial parts of this plant. The presence of phenolic glycosides and the α- and β-pinene-rich essential oils in A. chamaepitys may provide some rationale for the traditional medicinal uses of this species in Iran.

【 授权许可】

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