Chemical composition and cytotoxic activity of the essential oil from the leaves of Casearia lasiophylla

Revista Brasileira de Farmacognosia

Marcos José Salvador²  João Ernesto De Carvalho¹  Alberto Wisniewski-jr¹  Candida A. L. Kassuya¹  Élide P. Santos¹  Dilamara Riva¹  Maria Élida Alves Stefanello¹ 

[1] ,Universidade Estadual de Campinas Instituto de Biologia Departamento de Biologia Vegetal,Brazil

关键词: Casearia lasiophylla;    Salicaceae;    essential oil;    germacrene D;    β-caryophyllene;    antitumor;   

DOI  :  10.1590/S0102-695X2011005000073

来源: SciELO

【 摘 要 】

The essential oil obtained by hydrodistillation from fresh leaves of Casearia lasiophylla Eichler, Salicaceae, was analyzed by gas capillary (GC/FID and GC/MS). The cytotoxicity of the leaves essential oil was tested in vitro againstU251 (glioma), UACC-62 (melanoma), MCF-7 (breast), NC1-ADR/RES (ovarian-resistant), NCI-H460 (lung), PC03 (prostate), OVCAR-3 (ovarian), HT-29 (colon) and K562 (leukemia) human cancer cells and against VERO (no cancer cell). The yield of oil was 0.02%. Fifty two compounds were identified, representing 87.1% of the total of the oil. The main components were identified as germacrene D (18.6%), β-caryophyllene (14.7%), δ-cadinene (6.2%), and α-cadinol (5.4%). The oil exhibited antiproliferative activity against all cell lines (TGI<100 µg/mL), with exception of NCI-H460 cell line (TGI 191.31 µg/mL). The highest activity was observed against UACC-62 (TGI 7.30 µg/mL), and K562 (TGI 7.56 µg/mL) cell lines. The observed activity could be related to high content of germacrene D and β-caryophyllene, compounds known as cytotoxic.

【 授权许可】

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