Isolation of syringaldehyde from Mikania laevigata medicinal extract and its influence on the fatty acid profile of mice

Revista Brasileira de Farmacognosia

Ana Paula D. Pedroso¹  Sheila C. Santos¹  Ana A. Steil¹  Francisco Deschamps²  Andersson Barison²  Francinete Campos²  Maique W. Biavatti¹ 

[1] ,Universidade do Vale do Itajaí Núcleo de Investigações Químico-Farmacêuticas Itajaí SC ,Brazil

关键词: Mikania laevigata;    Asteraceae;    kaurenoic acid;    syringaldehyde;    arachidonic acid;    docosahexaenoic acid;    Mikania laevigata;    Asteraceae;    ácido caurenóico;    siringaldeído;    ácido araquidônico;    ácido docosahexaenóico;   

DOI  :  10.1590/S0102-695X2008000100013

来源: SciELO

【 摘 要 】

The Mikania genus is widely known as "guaco" and is used to treat fever, rheumatism, flu and respiratory diseases. Our previous work evidenced the synergism among M. laevigata extract components to produce desirable effects, and included the coumarin precursor, o-coumaric acid as marker. Many Mikania species are producers of ent-kaurene diterpenes which presented antiespasmodic and relaxant activities on smooth muscle. Seeking to standardize the guaco extract, which is registered in the Brazilian Pharmacopoea, this paper deals with the determination of kaurenoic acid through LC-PDA and the isolation through LC of syringaldehyde. Kaurenoic acid was not found in the extract, and syringaldehyde is one of the major compounds of pharmacopoeal extract, together with coumarin and o-coumaric acid. Samples from the lung and liver of Balb-C isogenic allergic pneumonitis bearing mice, treated with the same extract, were analyzed through GC-FID, and the fatty acid content was determined and analyzed. The results obtained by measuring the arachidonic acid (ARA) and docosahexaenoic acid (DHA) in the liver and lung of treated animals demonstrated that the fatty acid composition is distinct in both tissues, and that in the liver, only the DHA was altered as a result of the treatments. DHA is absent in the lung and in both organs, no significant difference in ARA production was observed. The aqueous extract, coumarin and o-coumaric acid stimulated DHA synthesis in the liver (p < 0.05).

【 授权许可】

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