Antibiotic, cytotoxic and enzyme inhibitory activity of crude extracts from Brazilian marine invertebrates

Revista Brasileira de Farmacognosia

Mirna H. R. Seleghim²  Simone P. Lira²  Miriam H. Kossuga²  Tatiana Batista²  Roberto G. S. Berlinck²  Eduardo Hajdu¹  Guilherme Muricy¹  Rosana M. Da Rocha¹  Gislene G. F. Do Nascimento¹  Marcio Silva¹  Eli F. Pimenta¹  Otávio H. Thiemann¹  Glaucius Oliva¹  Bruno C. Cavalcanti¹  Claudia Pessoa¹  Manoel O. De Moraes¹  Fabio C. S. Galetti¹  Celio L. Silva¹  Ana O. De Souza¹  Solange Peixinho¹ 

[1] ,Universidade de São Paulo Instituto de Química de São Carlos São Carlos SP ,Brazil

关键词: Biological screening;    marine sponges;    ascidians;    bryozoans;    octocorals;    antibacterial;    cytotoxic;    antimycobacterial;    enzymatic inhibitor;    Triagem biológica;    esponjas marinhas;    ascídias;    briozoários;    octocorais;    antibacteriano;    citotóxico;    antimicobacteriano;    inibidor enzimático;   

DOI  :  10.1590/S0102-695X2007000300002

来源: SciELO

【 摘 要 】

Herein we present the results of a screening with 349 crude extracts of Brazilian marine sponges, ascidians, bryozoans and octocorals, against 16 strains of susceptible and antibiotic-resistant bacteria, one yeast (Candida albicans), Mycobacterium tuberculosis H37Rv, three cancer cell lines MCF-7 (breast), B16 (murine melanoma ) and HCT8 (colon), and Leishmania tarentolae adenine phosphoribosyl transferase (L-APRT) enzyme. Less than 15% of marine sponge crude extracts displayed antibacterial activity, both against susceptible and antibiotic-resistant bacteria. Up to 40% of marine sponge crude extracts displayed antimycobacterial activity against M. tuberculosis H37Rv. Cytotoxicity was observed for 18% of marine sponge crude extracts. Finally, less than 3% of sponge extracts inhibited L-APRT. Less than 10% of ascidian crude extracts displayed antibacterial activity. More than 25% of ascidian crude extracts were active against M. tuberculosis and the three cancer cell lines. Only two crude extracts from the ascidian Polysyncraton sp. collected in different seasons (1995 and 1997) displayed activity against L-APRT. Less than 2% of bryozoan and octocoral crude extracts presented antibacterial activity, but a high percentage of crude extracts from bryozoan and octororal displayed cytotoxic (11% and 30%, respectively) and antimycobacterial (60%) activities. The extract of only one species of bryozoan, Bugula sp., presented inhibitory activity against L-APRT. Overall, the crude extracts of marine invertebrates herein investigated presented a high level of cytotoxic and antimycobacterial activities, a lower level of antibacterial activity and only a small number of crude extracts inhibited L-APRT. Taxonomic analysis of some of the more potently active crude extracts showed the occurrence of biological activity in taxa that have been previously chemically investigated. These include marine sponges belonging to genera Aaptos, Aplysina, Callyspongia, Haliclona, Niphates, Cliona, Darwinella, Dysidea, Ircinia, Monanchora and Mycale, ascidians of the genera Didemnum, Aplidium, Botrylloides, Clavelina, Polysyncraton and Symplegma, the bryozoan Bugula sp. and octocorals of the genera Carijoa and Lophogorgia. The subsequent chemical investigation of some of the active extracts led to the isolation of several new biologically active secondary metabolites. Our results are in agreement with previous screening programs carried out abroad, that showed a high percentage of bioactive extracts from Porifera, Ascidiacea, Cnidaria and Bryozoa.

【 授权许可】

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