Uso do Random Amplified Polymorphic DNA (RAPD) no estudo populacional do Triatoma brasiliensis Neiva, 1911

Cadernos de Saúde Pública

Érika C. Borges²  Alvaro J. Romanha¹  Liléia Diotaiuti² 

[1] ,Fundação Oswaldo Cruz Centro de Pesquisas René Rachou Laboratório de Triatomíneos e Epidemiologia da Doença de ChagasBelo Horizonte MG ,Brasil

关键词: Chagas Disease;    Triatominae;    Insect Vectors;    RAPD;    Doença de Chagas;    Triatominae;    Insetos Vetores;    RAPD;   

DOI  :  10.1590/S0102-311X2000000800012

来源: SciELO

【 摘 要 】

We evaluated the genetic variability of Triatoma brasiliensis, the main vector of Chagas disease in Northeast Brazil, using specimens from three populations. Regions of genomic DNA were amplified by RAPD (Random Amplified Polimorphic DNA), using two primers. The products were visualized after polyacrylamide gel electrophoresis followed by silver staining. A dendrogram constructed through the Dice similarity coefficient allowed for separation of the tested specimens into three distinct groups. The populations captured in areas from Ceará State showed similar profiles, but different from that captured in Piauí State. Our results indicate that RAPD can be used successfully in triatomine studies and suggest the presence of genetic variability between different populations of T. brasiliensis.

【 授权许可】

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