Evaluation of the PetrifilmTM and TEMPO® systems and the conventional method for counting microorganisms in pasteurized milk

Food Science and Technology (Campinas)

Andréia Cirolini¹  Andressa Mara Baseggio¹  Marília Miotto¹  Roberta Juliano Ramos¹  Cristhiane Stecanella De Oliveira Cattani¹  Cleide Rosana Werneck Vieira¹ 

[1] ,Federal University of Santa Catarina Center for Agricultural Sciences Department of Food Science and TechnologyFlorianópolis SC ,Brazil

关键词: alternative methods;    milk;    microbial contamination;    conventional methods;   

DOI  :  10.1590/S0101-20612013000400026

来源: SciELO

【 摘 要 】

New microbiological methods have been developed and commercialized, but their performance must be guaranteed. The aim of the present study was to evaluate the PetrifilmTM and TEMPO® systems compared to the conventional method for counting microorganisms in pasteurized milk. A total of 141 samples of pasteurized milk were analyzed by counting mesophilic aerobic, Coliforms at 35 ºC, Coliforms at 45 ºC, and Escherichia coli microorganisms. High correlation was found between the methods for counting Coliforms at 35 ºC, but low correlation was found for counting mesophilic aerobic, Coliforms at 45 ºC, and Escherichia coli. No significant statistical difference was found among the three methods for counting Coliforms at 35 ºC; however, the mean counts of mesophilic aerobic, Coliforms at 45 ºC, and Escherichia coli showed significant statistical difference. PetrifilmTM and TEMPO® systems had satisfactory results for Coliforms at 35 ºC in pasteurized milk but low performance for mesophilic aerobic, Coliforms at 45 ºC and Escherichia coli.

【 授权许可】

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